Near-optimal RNA-Seq quantification with kallisto

Standard

“The quantification of 78.6 million [RNAseq] reads takes 14 minutes on a standard desktop using a single CPU core.”

Currently the hot-topic on twitter, this new method of RNAseq quantification using psuedoalignment looks incredible, and could revolutionise our approach. Lior Patcher describes the process himself here, the arxiv here, and get to the github here.

kallisto-timings

Source: https://liorpachter.wordpress.com/2015/05/10/near-optimal-rna-seq-quantification-with-kallisto/